The content and ratio of plastid pigments in the needles of the platycladus orientalis during introduction

The relevance of the work is due to the high need for a theoretical justification for optimizing the range of urban green spaces and technologies for their creation. The aim of the research is to establish the pigment composition of the needles of the Platycladus orientalis or Oriental thuja (Platycladus orientalis (L.) Franco) when introduced into the conditions of the Nizhny Novgorod region (Russia) and the Almaty region (Kazakhstan). The object of the study was 1-year-old needles of biota harvested at the collection sites of Nizhny Novgorod State Agrotechnological University (Nizhny Novgorod, Russia) and Kazakh National Agrarian Research University (Almaty, Kazakhstan). The geographical coordinates of the locations are 56°14'30" N, 43°57'16" E and 43°14'31" N, 76°57'7" E, the absolute height is 141 m and 872 m, respectively. The methodological approach assumed compliance with the principle of the only logical difference and the requirements for typicality, suitability, reliability, optimality and expediency of the experience. The methodology is based on field and laboratory studies. The content of chlorophyll-a, chlorophyll-b and carotenoids was detected with a SF-2000 spectrophotometer with GRASS GIS 7.6.1 / QGIS 3.4 software. It was used to record absorption maxima for the above pigments at wavelengths: 665 nm, 649 nm, 452.5 nm. The heterogeneity of the pigment composition of the leaf apparatus of the accounting plants was recorded for all the tested indicators. The differences were noted both between the compared experimental sites and at the level of individual phenotypic variability within the boundaries of each of them. The average values of chlorophyll-a content were established, which ranged from 0.70±0.024 mg/g to 0.92±0.028 mg/g (Russian location) and from 0.86±0.017 mg/g to 0.95±0.042 mg/g (Kazakhstan location). The presence of chlorophyll-b in the conifers of plants in the surveyed areas was determined: from 0.35±0.018 mg/g to 0.71±0.081 mg/g in the Russian location and from 1.13±0.082 mg/g to 1.53±0.085 mg/g (tree 4) in Kazakhstan. The presence of carotenoids was detected: from 0.32±0.008 mg/g to 0.37±0.012 mg/g and from 0.24±0.021 mg/g to 0.38±0.032 mg/g in the Russian and Kazakh locations, respectively.

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