Accumulation of spare fats by the tissues of the shoot of common ash in the conditions of the Nizhny Novgorod region

The fat content in annual shoots of common ash was assessed. The objects of the study were trees located on the territory of the nature monument of regional importance Raspberry Ridge in the Nizhny Novgorod region. They are allocated according to the principle of typicality, their total number is 12. The test site had coordinates: 56°24'07"N 43°95'44" E. The work took into account the principle of the only logical difference and the key requirements: suitability, typicality and expediency of the experience. The study was conducted using field, stationary and laboratory methods. From each reference tree, 20 normally developed, intact annual shoots were selected in the periphery of a well-lit area of the middle tier of the crown. The primary sampling unit is represented by a one-time measurement of each of the tested parameters of all repetitions of the experiment. In our case, for each of the 12 tested trees, for each of the 8 tissues analyzed in them, accounting zones and their total index, 20 score fixes were performed. Accordingly, the volume of the database covered by the analysis amounted to 1920 data units. Cross sections were obtained from the central part of each shoot and examined using a Micmed-2 microscope using color histochemistry methods. The drug Sudan-III was used to detect fats. Their concentration in the tissues of the shoots was measured in conditional points on a scale that we developed. The variability of the fat content in the tissues of its shoots has been established. The amount of accumulated reserve nutrients in different tissues is not the same and has a significant variation in values between the accounting trees. The differences are most pronounced in the total fat content in the shoot tissues. The highest content was found in tree  No. 10 – 20.00± 0.68 points, and the lowest in tree No. 5 – 10.70± 0.63 points.

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