Preview

Conifers of the boreal area

Advanced search

Viability, fertility and optimal conditions for long-term storage of Siberian dwarf pine (Pinus pumila)

https://doi.org/10.53374/1993-0135-2025-1-27-34

Abstract

The viability and fertility of pollen play a key role in the development of the plant organism. Pollen represents a critical stage in the plant life cycle, as the viability of pollen is crucial for the effective sexual reproduction of plants. Pollen quality is assessed based on its viability. In this study, the pollen of Siberian dwarf pine (Pinus pumila) collected from shrubs growing in the Far North was studied. In order to carry out breeding work to obtain new promising varieties, it is often necessary to preserve viable pollen. For these purposes, a study was conducted to determine the most optimal shelf life of viable pollen for further use. It has been found that Siberian dwarf pine pollen can maintain its viability for a long time with a slight loss of germination ability. Anomalies in the development of pollen grains were also revealed, which indicates the ecological situation of the places where the object of research grows.  

About the Author

I. S. Sheveleva
Siberian Federal University
Russian Federation

79, Svobodny ave., Krasnoyarsk, 660041



References

1. Bazhina Ye. V., Kvitko O. V., Muratova Ye. N. Meyoz pri mikrosporogeneze i zhiznesposobnost' pyl'tsy u pikhty sibirskoy v srednegor'ye Vostochnogo Sayana // Lesovedeniye. 2007. № 1. S. 57–64.

2. Bazhina Ye. V., Sedayeva M. I. Zhiznespoobnost' pyl'tsy nekotorykh vidov Picea (Pinaceae) v usloviyakh Krasnoyarska // Botanicheskiy zhurn. 2017. T. 102. № 6. S. 768–779.

3. Municipal formation “Bilibinsky municipal district” : official. website. The Chukotka Autonomous Region, Bilibino. URL: https://www.bilchao.ru/.

4. Vladimirova O. S., Muratova Ye. N., Sedayeva M. I. Pyl'tsa yeli sibirskoy, proizrastayushchey v razlichnykh ekologicheskikh usloviyakh // Khvoynꞏyye boreal'noy zony. 2008. T. 25. № 1–2. S. 98–102.

5. Kalashnik N. A., Yasoviyeva S. M., Presnukhina L. P. Anomalii pyl'tsy khvoynykh vidov derev'yev pri promyshlennom zagryaznenii na Yuzhnom Urale // Lesovedeniye. 2008. № 2. S. 33–40.

6. Tret'yakova I. N., Novoselova N. V., Cherepov- skiy Yu. N. (2004) Osobennosti embrional'nogo razvitiya u kedra sibirskogo (Pinus sibirica Du Tour) s odnoletnim tsiklom razvitiya zhenskoy shishki v gorakh Zapadnogo Sayana. Fiziologiya rasteniy 51(1): 134–141.

7. Tret'yakova I. N., Izhboldina M. V. (2009) Induktsiya somaticheskogo embriogeneza u kedra sibirskogo. Lesovedeniye, 5: 43–49 [Tret’yakova I. N., Izhboldina M. V. (2009) Induction of Siberian pine somatic embryogenesis. Russian Journal of Forest Science [Lesovedenie], 5: 43–49.

8. Arista M., Talavera S. Pollen Dispersal Capacity and Pollen Viability of Abies pinsapo Boiss. // Silvae Genetica. 1994. Vol. 43. P. 155–158.

9. Bazhina E. Siberian fir seed productivity in V. N. Sukachev Institute of Forest Arboretum, Russia // Proceedings of the EuroGard VII Congress European Botanic gardens in the decade on biodiversity challenges and responsibilities in the count-down towards 2020 / Eds. Joly E., Larpin D., Delmas V., Carmine B. Paris: BGCI, 2018. P. 312–32.

10. Bellani L. M., Pacini E., Franchi G. G. (1985) In vitro pollen grain germination and starch content in species with different reproductive cycle. I. Lycopersicum peruvianum Mill. Acta Botanica Neerlandica 34: 59–64.

11. Caron G. E., Powell G. R. (1995) Pollen sizing in Jack Pine (Pinus banksiana Lamb.) with a hemocy-tometer. Silvae Genetica 44: 96–103.

12. Christiansen H. On the effect of low temperature on meiosis and pollen fertility in Larix decidua Mill. // Silvae Genet. 1960. B. 9. Hf. 3. S. 72–78.

13. Christiansen H. On the development of pollen and the fertilization mechanism of Picea abies (L.) Karst. // Silvae Genetica. 1972. Vol. 21. P. 5.

14. Clifford E. Ahlgren, Isabel F. Ahlgren Forest Science, 1978, Vol. 24, Issue 1, March, Pages 100–102.

15. Dawkins M. D., Owens J. N. In vitro and in vivo pollen hydration, germination, and pollen-tube growth in white spruce, Picea glauca (Moench) Voss. // International J. Plant Sciences. 1993. Vol. 154. P. 506– 521.

16. Delph L. F., Johannsson M. H., Stephenson A. G. How environmental factors affect pollen performance: ecological and evolutionary perspectives // Ecology. 1997. Vol. 78. P. 1632.

17. Doyle J. H., Verhoeven R. L., Bester C, Wingfield B. D., Botha A.-M. (2002) Germ-furrow morphology and storage conditions determine the degree of viability of Pinus caribaea pollen. South African Journal of Botany 68: 457–463.

18. Friedman W. E., Floyd S. K. The Origin of Flowering Plants and Their Reproductive Biology // Evolution. 2001. Vol. 55. P. 217–231.

19. Hak O., J. H. Russell. 2004. Environmental effects on yellow-cedar pollen quality. For. Gen. Council BC. Ext. Note 5.

20. Jett J. B., Bramlett D. L., Webber J. E., Eriksson U. (1993) Pollen collection, storage and testing. In: Bramlett DL (ed) Advances in Pollen Management. Agriculture Handbook No. 698. United States Department of Agriculture, Washington DC, pp. 41–46.

21. Moody W. R., Jett J. B. (1990) Effects of pollen viability and vigor on seed production of loblolly pine. Southern Journal of Applied Forestry 14: 33–38.

22. Nikkanen T., Aronen T., Häggman H., Venäläinen M. Variation in pollen viability among Picea abies genotypes – potential for unequal paternal success // Theoretical and Applied Genetics. 2000. Vol. 101. P. 511–518.

23. Pardi M. L., Viegi L., Renzoni G. C., Franchi G. G., Pacini E. (1996) Effects of acidity on the insoluble polysaccharide content of Pinus pinea L. and Pinus pinaster Aiton. Grana 35: 240–247.

24. Rana P. K., Kumar P., Singhal V. K. Spindle irregularities, chromatin transfer, and chromatin stickiness during male meiosis in Anemone tetrasepala (Ranunculaceae) // Turkish J. Botany. 2013. № 37. P. 167–176.

25. Roggen H. P. J. R. (1967) Changes in enzyme activities during the progame phase in Petunia hybrida. Acta Botanica Neerlandica 16: 1–31.

26. Runions C. J., Owens J. N. Sexual reproduction in interior spruce (Pinaceae). I. Pollen germination to archegonial maturation // International J. Plant Sciences. 1999. Vol. 160. P. 631–640.

27. Singh H. Embryology of gymnosperms. Berlin: Gerbruder Borntraeger, 1978. 302 p.

28. Siregar I. Z., Sweet G. B. (2000) The impact of extraction and storage conditions on the viability of radiata pine pollen. Silvae Genetica 49: 10–14.

29. Van Bilsen D. G. J. L., Van Roekel T., Hoekstra F. A. (1994) Declining viability and lipid degeneration during pollen storage. Sexual Plant Reproduction 7: 303–310.

30. Willemse M. T. M. (1968) Development of the micro- and macrogametophyte of Pinus silvestris L. Acta Botanica Neerlandica 17: 330–331.

31. Wright J. W. (1976) Introduction to Forest Genetics. Academic Press, Inc. New York.


Review

For citations:


Sheveleva I.S. Viability, fertility and optimal conditions for long-term storage of Siberian dwarf pine (Pinus pumila). Conifers of the boreal area. 2025;43(1):27–34. (In Russ.) https://doi.org/10.53374/1993-0135-2025-1-27-34

Views: 10


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 1993-0135 (Print)