Cultivated micromycetes of peat mounds of tundra woodlands of Western Taimyr
https://doi.org/10.53374/1993-0135-2025-1-20-26
Abstract
The biodiversity and biological features of cultivated microscopic fungi living in peat mounds of the tundra woodlands of Western Taimyr are studied. The species composition and vertical distribution of the dominant psychrophilic species at different depths (0.1; 1; 2; 4 m) were determined; identical species were identified at different depths. The isolates were identified based on a combination of morphological, cultural, and molecular genetic features. The growth parameters of the dominant species in the range of 12–16 ºC and the activity of their redox and hydrolytic enzymes were studied. A collection of fast-growing psychrophilic strains with a radial growth rate from 5 to 24 mm/day at 12 ºC has been formed, which is of practical interest for screening producers of enzyme preparations effective at low temperatures.
About the Authors
I. I. Jalolov
Reshetnev Siberian State University of Science and Technology; V. N. Sukachev Institute of Forest SB RAS – a separate division of the FRC KCS SB RAS (IF SB RAS)
Russian Federation
Russian Federation
31, Krasnoyarskii rabochii prospekt, Krasnoyarsk, 660037
50/28, Akademgorodok St., Krasnoyarsk, 660036
Yu. A. Litovka
Reshetnev Siberian State University of Science and Technology; V. N. Sukachev Institute of Forest SB RAS – a separate division of the FRC KCS SB RAS (IF SB RAS); Federal Research Center Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
31, Krasnoyarskii rabochii prospekt, Krasnoyarsk, 660037
50/28, Akademgorodok St., Krasnoyarsk, 660036
50, Akademgorodok St., Krasnoyarsk, 660036
N. V. Fomina
Reshetnev Siberian State University of Science and Technology; V. N. Sukachev Institute of Forest SB RAS – a separate division of the FRC KCS SB RAS (IF SB RAS)
Russian Federation
Russian Federation
31, Krasnoyarskii rabochii prospekt, Krasnoyarsk, 660037
50/28, Akademgorodok St., Krasnoyarsk, 660036
A. A. Timofeev
V. N. Sukachev Institute of Forest SB RAS – a separate division of the FRC KCS SB RAS (IF SB RAS); Federal Research Center Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
50/28, Akademgorodok St., Krasnoyarsk, 660036
50, Akademgorodok St., Krasnoyarsk, 660036
M. E. Arkanova
Reshetnev Siberian State University of Science and Technology
Russian Federation
Russian Federation
31, Krasnoyarskii rabochii prospekt, Krasnoyarsk, 660037
S. S. Poznukhova
Reshetnev Siberian State University of Science and Technology; V. N. Sukachev Institute of Forest SB RAS – a separate division of the FRC KCS SB RAS (IF SB RAS)
Russian Federation
Russian Federation
31, Krasnoyarskii rabochii prospekt, Krasnoyarsk, 660037
50/28, Akademgorodok St., Krasnoyarsk, 660036
K. A. Miroshnikova
Federal Research Center Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
50, Akademgorodok St., Krasnoyarsk, 660036
I. N. Pavlov
Reshetnev Siberian State University of Science and Technology; V. N. Sukachev Institute of Forest SB RAS – a separate division of the FRC KCS SB RAS (IF SB RAS)
Russian Federation
Russian Federation
31, Krasnoyarskii rabochii prospekt, Krasnoyarsk, 660037
50/28, Akademgorodok St., Krasnoyarsk, 660036
References
1. Turetsky, M. R. et al. Short term response of methane fluxes and methanogen activity to water table and soil warming manipulations in an Alaskan peatland. Journal of Geophysical Research: Biogeosciences, 2008, 113(G3). https://doi.org/10.1029/2007JG000496.
2. Thormann, M. N. Diversity and function of fungi in peatlands: A carbon cycling perspective. Canadian Journal of Soil Science, 2006, 86(2), 281–293. https://doi.org/10.4141/S05-082.
3. Vasilenko O.V. i dr. Analiz ekspressii genov u gribov – ekstremofilov Pseudogymnoascus pannorum s ispol'zovaniyem RNA - seq dlya izucheniya strategiy vyzhivaniya v neblagopriyatnykh usloviyakh sredy // Biokhimiya, fiziologiya i biosfernaya rol' mikroorganizmov: materialy Pervoy Pushchinskoy shkola-konferentsiya. Pushchino. 2014. S.132–134.
4. Weedon, J. T. et al. Temperature sensitivity of peatland C and N cycling: Does substrate supply play a role? Soil Biology and Biochemistry, 2013, 61, 109–120. https://doi.org/10.1016/j.soilbio.2013.02.026.
5. Margesin, R.; Miteva, V. Diversity and ecology of psychrophilic microorganisms. Research in Microbiology, 2011, 162(3), 346–361. https://doi.org/10.1016/j.resmic.2010.12.004.
6. Turunen, J. et al. Estimating carbon accumulation rates of undrained mires in Finland–application to boreal and subarctic regions. The Holocene, 2002, 12(1), 69–80.
7. Peay, K. G.; Kennedy, P. G.; Talbot, J. M. Dimensions of biodiversity in the Earth’s mycobiome. Nature Reviews Microbiology, 2016, 14(7), 434–447.
8. Koch, A. M. et al. A field method for sampling soil biodiversity. Applied Soil Ecology, 2004, 26(2), 181–190.
9. Gittel, Antje et al. Site- and horizon-specific patterns of microbial community structure and enzyme activities in permafrost-affected soils of Greenland. Frontiers in Microbiology 5, 2014.
10. White, T. J. et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols: a guide to methods and applications. Academic Press, 1990, 315–322.
11. Vermelho, Alane & Couri, Sonia. Methods to Determine Enzymatic Activity. Bentham Science Publishers, 2013, 332.
12. Margesin, R.; Miteva, V. Diversity and ecology of psychrophilic microorganisms. Research in Microbiology, 2011, 162(3), 346–361.
13. Inisheva L. I., Ivleva S. N., Shcherbakova T. A. Rukovodstvo po opredeleniyu fermentativnoy aktivnosti torfyanykh pochv i torfov. Tomsk: Izd-vo Tom. Un-ta. 2003. 122 s.
14. Khaziyev F. Kh. Metody pochvennoy enzimologii. M.: Nauka, 2005. 252 s.
15. Martorell, M. M. et al. Biodiversity and Enzymes Bioprospection of Antarctic Filamentous Fungi. Antarctic Science, 2019, 31(1): 3–12. doi: 10.1017/S0954102018 000421.
16. Kasana, R. C. et al. A Rapid and Easy Method for the Detection of Microbial Cellulases on Agar Plates Using Gram’s Iodine. Current Microbiology, 2008, 57, 503–507.
17. Dzhalolov, I. I. Izucheniye mikromitsetov, vydelennykh iz poverkhnostnogo sloya torfyanogo bugra pucheniya v Arktike / I. I. Dzhalolov // Molodyye uchen·yye v reshenii aktual'nykh problem nauki : Sbornik materialov Vserossiyskoy nauchno-prakticheskoy konferentsii studentov, aspirantov i molodykh uchenykh (s mezhdunarodnym uchastiyem), Krasnoyarsk, 18–19 aprelya 2024 goda. Krasnoyarsk : Federal'noye gosudarstvennoye byudzhetnoye obrazovatel'noye uchrezhdeniye vysshego obrazovaniya «Sibirskiy gosudarstvennyy universitet nauki i tekhnologiy imeni akademika M. F. Reshetneva», 2023. – S. 198–200.
18. Issledovaniye kul'tiviruyemykh mikromitsetov v torfyanykh bugrakh pucheniya v Arktike / I. I. Dzhalolov, Yu. A. Litovka, A. A. Timofeyev, I. N. Pavlov // Lesnoy i khimicheskiy kompleksy – problemy i resheniya : Sbornik materialov po itogam Vserossiyskoy nauchnoprakticheskoy konferentsii, Krasnoyarsk, 21 oktyabrya 2022 goda. – Krasnoyarsk : Federal'noye gosudarstvennoye byudzhetnoye obrazovatel'noye uchrezhdeniye vysshego obrazovaniya «Sibirskiy gosudarstvennyy universitet nauki i tekhnologiy imeni akademika M. F. Reshetneva», 2023. S. 34–36. EDN JIQEPY.
19. Mikromitsety Arktiki: bioraznoobraziye i biotekhnologicheskiy potentsial / I. I. Dzhalolov, Yu. A. Litovka, N. V. Fomina, I. N. Pavlov // III Yudakhinskiye 25–28 iyunya 2024 goda. Arkhangel'sk: KIRA, 2024. S. 225–227. EDN EOULOV.
20. Dzhalolov, I. I. Kul'tiviruyem·yye mikromitsety, vydelenn·yye iz glubinnykh obraztsov torfyanogo bugra pucheniya v Arktike // Molodyye uchen·yye v reshenii aktual'nykh problem nauki : Sbornik materialov Vserossiyskoy nauchno-prakticheskoy konferentsii studentov, aspirantov i molodykh uchenykh (s mezhdunarodnym uchastiyem), Krasnoyarsk, 20–21 aprelya 2023 goda. Krasnoyarsk : Federal'noye gosudarstvennoye byudzhetnoye obrazovatel'noye uchrezhdeniye vysshego obrazovaniya «Sibirskiy gosudarstvennyy universitet nauki i tekhnologiy imeni akademika M. F. Reshetneva», 2023. S. 283–285. EDN UWMWFO.
21. Gessler, N. N., Egorova, A. S.; Belozerskaya, T. A. Melanin pigments of fungi under extreme environmental conditions (Review). Appl Biochem Microbiol, 2014, 50, 105–113. https://doi.org/10.1134/S0003683814020094.
22. Hassan, N. et al. Psychrophilic and psychrotrophic fungi: a comprehensive review. Rev Environ Sci Biotechnol, 2016, 15, 147–172. https://doi.org/10.1007/s11157016-9395-9.
23. Robinson, C. H. Cold adaptation in Arctic and Antarctic fungi. New Phytologist, 2001, 151, 341–353.
24. Selbmann, L. et al. Fungi at the edge of life: Cryptoendolithic black fungi from Antarctic desert. Studies in mycology, 2005, 51. 1–32.
25. Clemmensen, K. E., et al. Roots and associated fungi drive long-term carbon sequestration in boreal forest. Science, 2013, 339(6127), 1615–1618.
26. Antipova, T .V. et al. The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products. Fermentation. 2023; 9(8):702. https://doi.org/10.3390/fermentation9080702.
Review
For citations:
Jalolov I.I., Litovka Yu.A., Fomina N.V., Timofeev A.A., Arkanova M.E., Poznukhova S.S., Miroshnikova K.A., Pavlov I.N. Cultivated micromycetes of peat mounds of tundra woodlands of Western Taimyr. Conifers of the boreal area. 2025;43(1):20–26. (In Russ.) https://doi.org/10.53374/1993-0135-2025-1-20-26
Views:
30
Email this article 