Reproduction of Pinus sibirica du tour on a genetic and breeding basis
Abstract
The article presents the results of variability of microsatellite loci of nuclear DNA of clone progeny of Siberian cedar pine trees 91/55, 94/58 and 100/64, growing on the grafting plantation of the Sentry district Forestry of the Educational and Experimental Forestry of the Reshetnev University (south of Central Siberia).
Based on the research results, 8 stable and reliable primers have been selected by polymerase chain reaction and electrophoresis. DNA genotypes of Siberian cedar pine samples have been identified. The probability of a random coincidence of alleles in unrelated genotypes was 1.5×10–6. 4 common DNA profiles were identified.
Keywords
Supporting agencies: The research was supported by Russian Federation President grant for young scientists – candidates of sciences MK-4099.2021.5.
About the Authors
Iu. E. Shcherbа
Reshetnev Siberian State University of Science and Technology
Russian Federation
Russian Federation
31, Krasnoyarskii rabochii prospekt, Krasnoyarsk, 660037
A. A. Ibe
Non-profit Partnership “Siberian forest certification center”
Russian Federation
Russian Federation
660036, г. Красноярск, ул. Академгородок, 50 а, 2
T. V. Sukhikh
Non-profit Partnership “Siberian forest certification center”
Russian Federation
Russian Federation
660036, г. Красноярск, ул. Академгородок, 50 а, 2
D. E. Kopchenko
Branch of the Federal State Institution “Roslesozashchita” – “Forest Protection Center of the Krasnoyarsk Territory”
Russian Federation
Russian Federation
50 a, 2, Akademgorodok str., Krasnoyarsk, 660036
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Review
For citations:
Shcherbа I.E., Ibe A.A., Sukhikh T.V., Kopchenko D.E. Reproduction of Pinus sibirica du tour on a genetic and breeding basis. Conifers of the boreal area. 2023;41(7):609-615.
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